Developed Formulas
  • Colonizes intestine for persistence

  • Provides an endogenous source of folic acid

Clinical test
In vitro test
Allergen Free
Real time stability
Preclinical test

Bifidobacterium lactis BA05 (DSM 18352)

Label Claim

Endogenous source of folic acid that ensures constant bioavailability

Effective nutraceutical combination

EFSA claim: contributes to maternal tissue growth during pregnancy; has a role in the process of cell division
EFSA claim: contributes to normal energyyielding. Metabolism; contributes to the maintenance of normal red blood cells; contributes to the normal metabolism of iron
Vitamin B6
EFSA claim: contributes to the regulation of hormonal activity
Vitamin B12
EFSA claim: contributes to the reduction of tiredness and fatigue

Scientific Rationale

Folate (vitamin B9) is an essential nutrient necessary for DNA replication and as a substrate in various enzymatic reactions involved in amino acid synthesis and vitamin metabolism. During pregnancy, the demand for folate escalates due to its critical role in fetal growth and development. Folic acid, a form of folate, is pivotal during a baby's development for the formation of the neural tube. Consequently, folic acid supplementation is vital during pregnancy to prevent neural tube defects, such as anencephaly and spina bifida, major birth defects of the baby’s brain and spine. Moreover, folate deficiency in mothers has been linked to anomalies like anemia and peripheral neuropathy [1].

The probiotic strain Bifidobacterium animalis subs. lactis BA05 has been chosen for its unique ability to produce 5-methyltetrahydrofolate (5-MTHF), the active form of folic acid [2]. This capability has been demonstrated in in vitro, preclinical, and clinical studies [3-4]. In healthy volunteers, 30 days of supplementation with BA05 significantly increased total fecal folates. BA05 was also confirmed to effectively colonize the gut environment [4]. Unlike traditional exogenous supplementation, utilizing 5-MTHF-producing bacteria as a supplement offers an endogenous, auto-regulated source of folic acid, ensuring a constant bioavailability of vitamin B9.


1. Greenberg JA, et al. Folic Acid supplementation and pregnancy: more than just neural tube defect prevention. Rev Obstet Gynecol. 2011 Summer;4(2):52-9.

2. Pompei A, et al. Folate production by bifidobacteria as a potential probiotic property. Appl Environ Microbiol. 2007 Jan;73(1):179-85. doi: 10.1128/AEM.01763-06

3. Pompei A, et al. Administration of folate-producing bifidobacteria enhances folate status in Wistar rats. J Nutr. 2007 Dec;137(12):2742-6. doi: 10.1093/jn/137.12.2742

4. Strozzi GP. and Mogna L. Quantification of folic acid in human faeces after administration of Bifidobacterium probiotic strains. Journal of Clinical Gastroenterology, 2008; 42:S179-S184. doi:10.1097/MCG.0b013e31818087d8